From cf035bd6c5da3f08bf0c7bb78a37173986f86847 Mon Sep 17 00:00:00 2001 From: ACCakut <7684542+ACCakut@users.noreply.github.com> Date: Wed, 3 May 2023 11:27:47 +0200 Subject: [PATCH] Added software sources --- 0_WGSPipeline/README.md | 16 +++++++++++++--- 1 file changed, 13 insertions(+), 3 deletions(-) diff --git a/0_WGSPipeline/README.md b/0_WGSPipeline/README.md index 141a78f..8b3471f 100644 --- a/0_WGSPipeline/README.md +++ b/0_WGSPipeline/README.md @@ -1,11 +1,21 @@ # About the scripts in this folder: - **0_DicIndex.sh** - - The dictionaries needed for the alignment steps are indexed and sorted. + - The dictionaries needed for the alignment steps are indexed and sorted. + - Programs used in the script + - BWA https://github.com/lh3/bwa + - samfold from https://github.com/samtools/samtools + - Picard https://github.com/broadinstitute/picard (requires Java) - **1_QCScript.sh** - Raw reads are trimmed and adapters are removed. The read quality is assessed before and after the trimming. + - Programs used in the script + - FastQC https://www.bioinformatics.babraham.ac.uk/projects/download.html#fastqc + - Trimmomatic https://github.com/usadellab/Trimmomatic - **2_CombiScriptSamtools.sh** - - The trimmed reads are aligned to the reference and variants are called. The coverage at each position is evaluated. - + - The trimmed reads are aligned to the reference and variants are called. The coverage at each position is evaluated. + - Programs used in the script + - bwa and samtools, see above + - BCFtools https://github.com/samtools/bcftools + - Bedtools 2 https://github.com/arq5x/bedtools2