diff --git a/src/CONST/index.ts b/src/CONST/index.ts
index 19b861bcaf5ff..2b4fa6fbde0d6 100644
--- a/src/CONST/index.ts
+++ b/src/CONST/index.ts
@@ -5813,6 +5813,8 @@ const CONST = {
         LINK: 'link',
         /** Use to identify a list of items. */
         LIST: 'list',
+        /** Use for a list of selectable options (single or multi-select). */
+        LISTBOX: 'listbox',
         /** Use for individual items within a list. */
         LISTITEM: 'listitem',
         /** Use for a list of choices or options. */
@@ -5821,6 +5823,8 @@ const CONST = {
         MENUBAR: 'menubar',
         /** Use for items within a menu. */
         MENUITEM: 'menuitem',
+        /** Use for selectable options within a listbox. */
+        OPTION: 'option',
         /** Use when no specific role is needed. */
         NONE: 'none',
         /** Use for elements that don't require a specific role. */
diff --git a/src/components/SelectionList/BaseSelectionList.tsx b/src/components/SelectionList/BaseSelectionList.tsx
index 025e6ca249751..9b8e7888b258d 100644
--- a/src/components/SelectionList/BaseSelectionList.tsx
+++ b/src/components/SelectionList/BaseSelectionList.tsx
@@ -27,6 +27,7 @@ import useSearchFocusSync from './hooks/useSearchFocusSync';
 import useSelectedItemFocusSync from './hooks/useSelectedItemFocusSync';
 import ListItemRenderer from './ListItem/ListItemRenderer';
 import type {ButtonOrCheckBoxRoles, DataDetailsType, ListItem, SelectionListProps} from './types';
+import {getListboxRole} from './utils/getListboxRole';
 
 const ANIMATED_HIGHLIGHT_DURATION =
     CONST.ANIMATED_HIGHLIGHT_ENTRY_DELAY +
@@ -550,6 +551,7 @@ function BaseSelectionList<TItem extends ListItem>({
                 <>
                     {!shouldHeaderBeInsideList && header}
                     <FlashList
+                        role={getListboxRole(canSelectMultiple)}
                         data={data}
                         renderItem={renderItem}
                         ref={listRef}
diff --git a/src/components/SelectionList/ListItem/BaseListItem.tsx b/src/components/SelectionList/ListItem/BaseListItem.tsx
index 59ef77d63452d..d59a897f9567a 100644
--- a/src/components/SelectionList/ListItem/BaseListItem.tsx
+++ b/src/components/SelectionList/ListItem/BaseListItem.tsx
@@ -84,8 +84,12 @@ function BaseListItem<TItem extends ListItem>({
 
     const shouldShowHiddenCheckmark = shouldShowRBRIndicator && !shouldShowCheckmark && !!item.canShowSeveralIndicators;
 
+    // For single-select lists, use role="option" with aria-selected so screen readers announce "selected"/"not selected".
+    // For multi-select (checkbox/radio), keep existing role and state.
+    const isSelectableOption = !canSelectMultiple && accessibilityRole !== CONST.ROLE.CHECKBOX && accessibilityRole !== CONST.ROLE.RADIO;
+    const effectiveRole = isSelectableOption ? CONST.ROLE.OPTION : accessibilityRole;
     const accessibilityState =
-        accessibilityRole === CONST.ROLE.CHECKBOX || accessibilityRole === CONST.ROLE.RADIO ? {checked: !!item.isSelected, selected: !!isFocused} : {selected: !!isFocused};
+        accessibilityRole === CONST.ROLE.CHECKBOX || accessibilityRole === CONST.ROLE.RADIO ? {checked: !!item.isSelected, selected: !!isFocused} : {selected: !!item.isSelected};
 
     return (
         <OfflineWithFeedback
@@ -139,13 +143,12 @@ function BaseListItem<TItem extends ListItem>({
                 onMouseLeave={handleMouseLeave}
                 tabIndex={accessible === false ? -1 : item.tabIndex}
                 wrapperStyle={pressableWrapperStyle}
-                testID={testID}
+                testID={`${CONST.BASE_LIST_ITEM_TEST_ID}${item.keyForList}`}
                 accessible={accessible}
-                role={accessible === false ? CONST.ROLE.PRESENTATION : accessibilityRole}
+                role={accessible === false ? CONST.ROLE.PRESENTATION : effectiveRole}
             >
                 <View
-                    testID={`${CONST.BASE_LIST_ITEM_TEST_ID}${item.keyForList}`}
-                    accessibilityState={{selected: !!isFocused}}
+                    testID={testID}
                     style={[
                         wrapperStyle,
                         isFocused &&
diff --git a/src/components/SelectionList/SelectionListWithSections/BaseSelectionListWithSections.tsx b/src/components/SelectionList/SelectionListWithSections/BaseSelectionListWithSections.tsx
index 545c43c2f0cf6..3c8afa0fe3706 100644
--- a/src/components/SelectionList/SelectionListWithSections/BaseSelectionListWithSections.tsx
+++ b/src/components/SelectionList/SelectionListWithSections/BaseSelectionListWithSections.tsx
@@ -13,6 +13,7 @@ import useSearchFocusSync from '@components/SelectionList/hooks/useSearchFocusSy
 import useSelectedItemFocusSync from '@components/SelectionList/hooks/useSelectedItemFocusSync';
 import ListItemRenderer from '@components/SelectionList/ListItem/ListItemRenderer';
 import type {ButtonOrCheckBoxRoles} from '@components/SelectionList/types';
+import {getListboxRole} from '@components/SelectionList/utils/getListboxRole';
 import Text from '@components/Text';
 import type {BaseTextInputRef} from '@components/TextInput/BaseTextInput/types';
 import useActiveElementRole from '@hooks/useActiveElementRole';
@@ -88,7 +89,6 @@ function BaseSelectionListWithSections<TItem extends ListItem>({
     const {isKeyboardShown} = useKeyboardState();
     const {safeAreaPaddingBottomStyle} = useSafeAreaPaddings();
     const triggerScrollEvent = useScrollEventEmitter();
-
     const paddingBottomStyle = !isKeyboardShown && !footerContent && safeAreaPaddingBottomStyle;
 
     const {flattenedData, disabledIndexes, itemsCount, selectedItems, initialFocusedIndex, firstFocusableIndex} = useFlattenedSections(sections, initiallyFocusedItemKey);
@@ -358,6 +358,7 @@ function BaseSelectionListWithSections<TItem extends ListItem>({
                 renderListEmptyContent()
             ) : (
                 <FlashList
+                    role={getListboxRole(canSelectMultiple)}
                     data={flattenedData}
                     renderItem={renderItem}
                     ref={listRef}
diff --git a/src/components/SelectionList/utils/getListboxRole/index.ts b/src/components/SelectionList/utils/getListboxRole/index.ts
new file mode 100644
index 0000000000000..f174324481d8a
--- /dev/null
+++ b/src/components/SelectionList/utils/getListboxRole/index.ts
@@ -0,0 +1,6 @@
+import type {GetListboxRole} from './types';
+
+const getListboxRole: GetListboxRole = () => undefined;
+
+// eslint-disable-next-line import/prefer-default-export
+export {getListboxRole};
diff --git a/src/components/SelectionList/utils/getListboxRole/index.web.ts b/src/components/SelectionList/utils/getListboxRole/index.web.ts
new file mode 100644
index 0000000000000..f20364a6872ea
--- /dev/null
+++ b/src/components/SelectionList/utils/getListboxRole/index.web.ts
@@ -0,0 +1,8 @@
+import type {Role} from 'react-native';
+import CONST from '@src/CONST';
+import type {GetListboxRole} from './types';
+
+const getListboxRole: GetListboxRole = (canSelectMultiple) => (!canSelectMultiple ? (CONST.ROLE.LISTBOX as Role) : undefined);
+
+// eslint-disable-next-line import/prefer-default-export
+export {getListboxRole};
diff --git a/src/components/SelectionList/utils/getListboxRole/types.ts b/src/components/SelectionList/utils/getListboxRole/types.ts
new file mode 100644
index 0000000000000..f78e7a8494844
--- /dev/null
+++ b/src/components/SelectionList/utils/getListboxRole/types.ts
@@ -0,0 +1,6 @@
+import type {Role} from 'react-native';
+
+type GetListboxRole = (canSelectMultiple: boolean) => Role | undefined;
+
+// eslint-disable-next-line import/prefer-default-export
+export type {GetListboxRole};
diff --git a/src/components/SelectionListWithSections/BaseListItem.tsx b/src/components/SelectionListWithSections/BaseListItem.tsx
index 87c5961003be6..71481960c11ac 100644
--- a/src/components/SelectionListWithSections/BaseListItem.tsx
+++ b/src/components/SelectionListWithSections/BaseListItem.tsx
@@ -81,8 +81,12 @@ function BaseListItem<TItem extends ListItem>({
     const defaultAccessibilityLabel = item.text === item.alternateText ? (item.text ?? '') : [item.text, item.alternateText].filter(Boolean).join(', ');
     const accessibilityLabel = item.accessibilityLabel ?? defaultAccessibilityLabel;
 
+    // For single-select lists, use role="option" with aria-selected so screen readers announce "selected"/"not selected".
+    // For multi-select (checkbox/radio), keep existing role and state.
+    const isSelectableOption = !canSelectMultiple && accessibilityRole !== CONST.ROLE.CHECKBOX && accessibilityRole !== CONST.ROLE.RADIO;
+    const effectiveRole = isSelectableOption ? CONST.ROLE.OPTION : accessibilityRole;
     const accessibilityState =
-        accessibilityRole === CONST.ROLE.CHECKBOX || accessibilityRole === CONST.ROLE.RADIO ? {checked: !!item.isSelected, selected: !!isFocused} : {selected: !!isFocused};
+        accessibilityRole === CONST.ROLE.CHECKBOX || accessibilityRole === CONST.ROLE.RADIO ? {checked: !!item.isSelected, selected: !!isFocused} : {selected: !!item.isSelected};
 
     const accessibleProps = accessible === false ? {accessible: false as const, role: undefined} : {accessibilityLabel, role: accessibilityRole};
 
@@ -117,6 +121,7 @@ function BaseListItem<TItem extends ListItem>({
                 // eslint-disable-next-line react/jsx-props-no-spreading
                 {...accessibleProps}
                 accessibilityState={accessibilityState}
+                role={effectiveRole}
                 isNested
                 hoverDimmingValue={1}
                 pressDimmingValue={item.isInteractive === false ? 1 : variables.pressDimValue}
@@ -134,11 +139,10 @@ function BaseListItem<TItem extends ListItem>({
                 onMouseLeave={handleMouseLeave}
                 tabIndex={item.tabIndex}
                 wrapperStyle={pressableWrapperStyle}
-                testID={testID}
+                testID={`${CONST.BASE_LIST_ITEM_TEST_ID}${item.keyForList}`}
             >
                 <View
-                    testID={`${CONST.BASE_LIST_ITEM_TEST_ID}${item.keyForList}`}
-                    accessibilityState={{selected: !!isFocused}}
+                    testID={testID}
                     style={[
                         wrapperStyle,
                         isFocused &&
diff --git a/src/components/SelectionListWithSections/BaseSelectionListWithSections.tsx b/src/components/SelectionListWithSections/BaseSelectionListWithSections.tsx
index 9b90b4a4d12c4..a446eeefe398b 100644
--- a/src/components/SelectionListWithSections/BaseSelectionListWithSections.tsx
+++ b/src/components/SelectionListWithSections/BaseSelectionListWithSections.tsx
@@ -10,6 +10,7 @@ import FixedFooter from '@components/FixedFooter';
 import OptionsListSkeletonView from '@components/OptionsListSkeletonView';
 import {PressableWithFeedback} from '@components/Pressable';
 import SectionList from '@components/SectionList';
+import {getListboxRole} from '@components/SelectionList/utils/getListboxRole';
 import Text from '@components/Text';
 import TextInput from '@components/TextInput';
 import useActiveElementRole from '@hooks/useActiveElementRole';
@@ -1044,6 +1045,7 @@ function BaseSelectionListWithSections<TItem extends ListItem>({
                         renderScrollComponent={renderScrollComponent}
                         removeClippedSubviews={removeClippedSubviews}
                         ref={listRef}
+                        role={getListboxRole(canSelectMultiple)}
                         sections={slicedSections}
                         stickySectionHeadersEnabled={false}
                         renderSectionHeader={(arg) => (