diff --git a/airflow/providers/amazon/aws/sensors/s3.py b/airflow/providers/amazon/aws/sensors/s3.py
index 40e31596d78c7..57c9393c0c583 100644
--- a/airflow/providers/amazon/aws/sensors/s3.py
+++ b/airflow/providers/amazon/aws/sensors/s3.py
@@ -86,7 +86,7 @@ def __init__(
     ):
         super().__init__(**kwargs)
         self.bucket_name = bucket_name
-        self.bucket_key = [bucket_key] if isinstance(bucket_key, str) else bucket_key
+        self.bucket_key = bucket_key
         self.wildcard_match = wildcard_match
         self.check_fn = check_fn
         self.aws_conn_id = aws_conn_id
@@ -125,7 +125,10 @@ def _check_key(self, key):
         return True
 
     def poke(self, context: Context):
-        return all(self._check_key(key) for key in self.bucket_key)
+        if isinstance(self.bucket_key, str):
+            return self._check_key(self.bucket_key)
+        else:
+            return all(self._check_key(key) for key in self.bucket_key)
 
     def get_hook(self) -> S3Hook:
         """Create and return an S3Hook"""
diff --git a/tests/providers/amazon/aws/sensors/test_s3_key.py b/tests/providers/amazon/aws/sensors/test_s3_key.py
index 8d560e2c82044..f0832d3df9a89 100644
--- a/tests/providers/amazon/aws/sensors/test_s3_key.py
+++ b/tests/providers/amazon/aws/sensors/test_s3_key.py
@@ -126,6 +126,33 @@ def test_parse_bucket_key_from_jinja(self, mock_head_object):
 
         mock_head_object.assert_called_once_with("key", "bucket")
 
+    @mock.patch("airflow.providers.amazon.aws.sensors.s3.S3Hook.head_object")
+    def test_parse_list_of_bucket_keys_from_jinja(self, mock_head_object):
+        mock_head_object.return_value = None
+        mock_head_object.side_effect = [{"ContentLength": 0}, {"ContentLength": 0}]
+
+        Variable.set("test_bucket_key", ["s3://bucket/file1", "s3://bucket/file2"])
+
+        execution_date = timezone.datetime(2020, 1, 1)
+
+        dag = DAG("test_s3_key", start_date=execution_date, render_template_as_native_obj=True)
+        op = S3KeySensor(
+            task_id="s3_key_sensor",
+            bucket_key="{{ var.value.test_bucket_key }}",
+            bucket_name=None,
+            dag=dag,
+        )
+
+        dag_run = DagRun(dag_id=dag.dag_id, execution_date=execution_date, run_id="test")
+        ti = TaskInstance(task=op)
+        ti.dag_run = dag_run
+        context = ti.get_template_context()
+        ti.render_templates(context)
+        op.poke(None)
+
+        mock_head_object.assert_any_call("file1", "bucket")
+        mock_head_object.assert_any_call("file2", "bucket")
+
     @mock.patch("airflow.providers.amazon.aws.sensors.s3.S3Hook.head_object")
     def test_poke(self, mock_head_object):
         op = S3KeySensor(task_id="s3_key_sensor", bucket_key="s3://test_bucket/file")