diff --git a/airflow-core/src/airflow/serialization/serialized_objects.py b/airflow-core/src/airflow/serialization/serialized_objects.py
index 680816a654555..d43a2375ef996 100644
--- a/airflow-core/src/airflow/serialization/serialized_objects.py
+++ b/airflow-core/src/airflow/serialization/serialized_objects.py
@@ -2507,6 +2507,23 @@ def serialize_dag(cls, dag: DAG) -> dict:
                 serialized_dag["has_on_success_callback"] = True
             if dag.has_on_failure_callback:
                 serialized_dag["has_on_failure_callback"] = True
+
+            # TODO: Move this logic to a better place -- ideally before serializing contents of default_args.
+            #   There is some duplication with this and SerializedBaseOperator.partial_kwargs serialization.
+            #   Ideally default_args goes through same logic as fields of SerializedBaseOperator.
+            if serialized_dag.get("default_args", {}):
+                default_args_dict = serialized_dag["default_args"][Encoding.VAR]
+                callbacks_to_remove = []
+                for k, v in list(default_args_dict.items()):
+                    if k in [
+                        f"on_{x}_callback" for x in ("execute", "failure", "success", "retry", "skipped")
+                    ]:
+                        if bool(v):
+                            default_args_dict[f"has_{k}"] = True
+                        callbacks_to_remove.append(k)
+                for k in callbacks_to_remove:
+                    del default_args_dict[k]
+
             return serialized_dag
         except SerializationError:
             raise
diff --git a/airflow-core/tests/unit/serialization/test_dag_serialization.py b/airflow-core/tests/unit/serialization/test_dag_serialization.py
index 40864f18ee795..c06e3a47ed2a7 100644
--- a/airflow-core/tests/unit/serialization/test_dag_serialization.py
+++ b/airflow-core/tests/unit/serialization/test_dag_serialization.py
@@ -4292,3 +4292,65 @@ def test_partial_kwargs_end_to_end_deserialization(self):
         assert "owner" in deserialized_task.partial_kwargs
         assert deserialized_task.partial_kwargs["retry_delay"] == timedelta(seconds=600)
         assert deserialized_task.partial_kwargs["owner"] == "custom_owner"
+
+
+@pytest.mark.parametrize(
+    ["callbacks", "expected_has_flags", "absent_keys"],
+    [
+        pytest.param(
+            {
+                "on_failure_callback": lambda ctx: None,
+                "on_success_callback": lambda ctx: None,
+                "on_retry_callback": lambda ctx: None,
+            },
+            ["has_on_failure_callback", "has_on_success_callback", "has_on_retry_callback"],
+            ["on_failure_callback", "on_success_callback", "on_retry_callback"],
+            id="multiple_callbacks",
+        ),
+        pytest.param(
+            {"on_failure_callback": lambda ctx: None},
+            ["has_on_failure_callback"],
+            ["on_failure_callback", "has_on_success_callback", "on_success_callback"],
+            id="single_callback",
+        ),
+        pytest.param(
+            {"on_failure_callback": lambda ctx: None, "on_execute_callback": None},
+            ["has_on_failure_callback"],
+            ["on_failure_callback", "has_on_execute_callback", "on_execute_callback"],
+            id="callback_with_none",
+        ),
+        pytest.param(
+            {},
+            [],
+            [
+                "has_on_execute_callback",
+                "has_on_failure_callback",
+                "has_on_success_callback",
+                "has_on_retry_callback",
+                "has_on_skipped_callback",
+            ],
+            id="no_callbacks",
+        ),
+    ],
+)
+def test_dag_default_args_callbacks_serialization(callbacks, expected_has_flags, absent_keys):
+    """Test callbacks in DAG default_args are serialized as boolean flags."""
+    default_args = {"owner": "test_owner", "retries": 2, **callbacks}
+
+    with DAG(dag_id="test_default_args_callbacks", default_args=default_args) as dag:
+        BashOperator(task_id="task1", bash_command="echo 1", dag=dag)
+
+    serialized_dag_dict = SerializedDAG.serialize_dag(dag)
+    default_args_dict = serialized_dag_dict["default_args"][Encoding.VAR]
+
+    for flag in expected_has_flags:
+        assert default_args_dict.get(flag) is True
+
+    for key in absent_keys:
+        assert key not in default_args_dict
+
+    assert default_args_dict["owner"] == "test_owner"
+    assert default_args_dict["retries"] == 2
+
+    deserialized_dag = SerializedDAG.deserialize_dag(serialized_dag_dict)
+    assert deserialized_dag.dag_id == "test_default_args_callbacks"