diff --git a/python/tvm/relax/frontend/onnx/onnx_frontend.py b/python/tvm/relax/frontend/onnx/onnx_frontend.py
index f3a5e9098de8..dfd092314cbc 100644
--- a/python/tvm/relax/frontend/onnx/onnx_frontend.py
+++ b/python/tvm/relax/frontend/onnx/onnx_frontend.py
@@ -2119,13 +2119,18 @@ def _impl_v18(cls, bb, inputs, attr, params):
 
         # Define relax implementation.
         if roi is not None:
-            roi = relax.op.concat(
-                [
-                    relax.op.strided_slice(roi, axes=[0], begin=[2], end=[ndims]),
-                    relax.op.strided_slice(roi, axes=[0], begin=[ndims + 2], end=[2 * ndims]),
-                ],
-                axis=0,
-            )
+            if isinstance(roi, relax.Constant):
+                roi = roi.data.numpy().tolist()
+            else:
+                roi = relax.op.concat(
+                    [
+                        relax.op.strided_slice(roi, axes=[0], begin=[2], end=[ndims]),
+                        relax.op.strided_slice(roi, axes=[0], begin=[ndims + 2], end=[2 * ndims]),
+                    ],
+                    axis=0,
+                )
+                # TODO The backend C++ func resize2d does not support dynamic ROI for now.
+                raise NotImplementedError("Dynamic ROI is not supported in resize2d for now.")
         else:
             roi = [0.0] * 4
 
diff --git a/python/tvm/relax/op/image/image.py b/python/tvm/relax/op/image/image.py
index 6bec22161dbc..afadbf35fb6b 100644
--- a/python/tvm/relax/op/image/image.py
+++ b/python/tvm/relax/op/image/image.py
@@ -104,6 +104,10 @@ def resize2d(
         roi = (0.0, 0.0, 0.0, 0.0)  # type: ignore
     elif isinstance(roi, float):
         roi = (roi, roi, roi, roi)  # type: ignore
+    elif isinstance(roi, (tuple, list)):
+        roi = tuple(val if isinstance(val, float) else float(val) for val in roi)
+    else:
+        raise NotImplementedError(f"Unsupported roi type {type(roi)}")
 
     if isinstance(size, (int, PrimExpr)):
         size = (size, size)
diff --git a/tests/python/relax/test_frontend_onnx.py b/tests/python/relax/test_frontend_onnx.py
index 0d532e07fc33..74d75f5abdd7 100644
--- a/tests/python/relax/test_frontend_onnx.py
+++ b/tests/python/relax/test_frontend_onnx.py
@@ -2359,8 +2359,34 @@ def verify_tile(in_shape, repeats, out_shape):
     verify_tile(x.shape, repeats, z_array.shape)
 
 
-def test_resize():
-    resize_node = helper.make_node("Resize", ["X", "", "scales"], ["Y"], mode="cubic")
+def _generate_roi_cases():
+    # Base case when with_roi is False
+    roi_list = [
+        pytest.param(False, None, id="no_roi"),
+    ]
+
+    # Valid when with_roi is True
+    roi_cases = [
+        [0.0, 0.0, 0.0, 0.0],
+        [0.0, 0.0, 1.0, 1.0],
+        [0.1, 0.1, 0.9, 0.9],
+        [0.2, 0.2, 0.8, 0.8],
+        [0.3, 0.3, 0.7, 0.7],
+        [0.4, 0.4, 0.6, 0.6],
+        [0.5, 0.5, 0.5, 0.5],
+        [0.1, 0.2, 0.9, 0.8],
+    ]
+    for roi in roi_cases:
+        roi_list.append(pytest.param(True, roi, id=f"roi_{'_'.join(str(x) for x in roi)}"))
+
+    return roi_list
+
+
+@pytest.mark.parametrize("with_roi, roi_list", _generate_roi_cases())
+def test_resize(with_roi, roi_list):
+    resize_node = helper.make_node(
+        "Resize", ["X", "roi" if with_roi else "", "scales"], ["Y"], mode="cubic"
+    )
 
     graph = helper.make_graph(
         [resize_node],
@@ -2370,6 +2396,11 @@ def test_resize():
         ],
         initializer=[
             helper.make_tensor("scales", TensorProto.FLOAT, [4], [1.0, 1.0, 2.0, 2.0]),
+            *(
+                [helper.make_tensor("roi", TensorProto.FLOAT, [4], [0.0, 0.0, 0.0, 0.0])]
+                if with_roi
+                else []
+            ),
         ],
         outputs=[
             helper.make_tensor_value_info("Y", TensorProto.FLOAT, [1, 3, 64, 64]),