diff --git a/src/libraries/Microsoft.Extensions.DependencyInjection/src/ServiceLookup/ConstantCallSite.cs b/src/libraries/Microsoft.Extensions.DependencyInjection/src/ServiceLookup/ConstantCallSite.cs
index 979db8cffcf907..1c488d95f9a1b1 100644
--- a/src/libraries/Microsoft.Extensions.DependencyInjection/src/ServiceLookup/ConstantCallSite.cs
+++ b/src/libraries/Microsoft.Extensions.DependencyInjection/src/ServiceLookup/ConstantCallSite.cs
@@ -10,7 +10,7 @@ internal sealed class ConstantCallSite : ServiceCallSite
         private readonly Type _serviceType;
         internal object? DefaultValue => Value;
 
-        public ConstantCallSite(Type serviceType, object? defaultValue, object? serviceKey = null) : base(ResultCache.None(serviceType), serviceKey)
+        public ConstantCallSite(Type serviceType, object? defaultValue, object? serviceKey = null) : base(ResultCache.None(serviceType, serviceKey), serviceKey)
         {
             _serviceType = serviceType ?? throw new ArgumentNullException(nameof(serviceType));
             if (defaultValue != null && !serviceType.IsInstanceOfType(defaultValue))
diff --git a/src/libraries/Microsoft.Extensions.DependencyInjection/src/ServiceLookup/ResultCache.cs b/src/libraries/Microsoft.Extensions.DependencyInjection/src/ServiceLookup/ResultCache.cs
index 8cf73ab099eb3f..6651ffce05e275 100644
--- a/src/libraries/Microsoft.Extensions.DependencyInjection/src/ServiceLookup/ResultCache.cs
+++ b/src/libraries/Microsoft.Extensions.DependencyInjection/src/ServiceLookup/ResultCache.cs
@@ -8,9 +8,9 @@ namespace Microsoft.Extensions.DependencyInjection.ServiceLookup
 {
     internal struct ResultCache
     {
-        public static ResultCache None(Type serviceType)
+        public static ResultCache None(Type serviceType, object? serviceKey = null)
         {
-            var cacheKey = new ServiceCacheKey(ServiceIdentifier.FromServiceType(serviceType), 0);
+            var cacheKey = new ServiceCacheKey(new ServiceIdentifier(serviceKey, serviceType), 0);
             return new ResultCache(CallSiteResultCacheLocation.None, cacheKey);
         }
 
diff --git a/src/libraries/Microsoft.Extensions.DependencyInjection/tests/DI.Tests/ServiceProviderValidationTests.cs b/src/libraries/Microsoft.Extensions.DependencyInjection/tests/DI.Tests/ServiceProviderValidationTests.cs
index 3feb626c175331..aa372ce748cd56 100644
--- a/src/libraries/Microsoft.Extensions.DependencyInjection/tests/DI.Tests/ServiceProviderValidationTests.cs
+++ b/src/libraries/Microsoft.Extensions.DependencyInjection/tests/DI.Tests/ServiceProviderValidationTests.cs
@@ -292,6 +292,35 @@ public void ScopeValidation_ShouldBeAbleToDistingushGenericCollections_WhenGetSe
             Assert.IsType<Baz2>(actual.Last());
         }
 
+        [Theory]
+        [InlineData(true)]
+        [InlineData(false)]
+        public void BuildServiceProvider_ValidateOnBuild_DoesNotThrow_WhenKeyedSingletonInstanceAndScopedShareServiceType(bool registerScopedFirst)
+        {
+            // Arrange
+            var serviceCollection = new ServiceCollection();
+            if (registerScopedFirst)
+            {
+                serviceCollection.AddScoped<IKeyedScopedCollisionService, KeyedScopedCollisionScopedService>();
+                serviceCollection.AddKeyedSingleton<IKeyedScopedCollisionService>("Key", new KeyedScopedCollisionSingletonService());
+            }
+            else
+            {
+                serviceCollection.AddKeyedSingleton<IKeyedScopedCollisionService>("Key", new KeyedScopedCollisionSingletonService());
+                serviceCollection.AddScoped<IKeyedScopedCollisionService, KeyedScopedCollisionScopedService>();
+            }
+
+            serviceCollection.AddSingleton<KeyedScopedCollisionOtherSingleton>();
+
+            // Act + Assert
+            using var serviceProvider = serviceCollection.BuildServiceProvider(new ServiceProviderOptions
+            {
+                ValidateScopes = true,
+                ValidateOnBuild = true
+            });
+            Assert.NotNull(serviceProvider);
+        }
+
         [Fact]
         public void GetService_DoesNotThrow_WhenScopeFactoryIsInjectedIntoSingleton()
         {
@@ -586,5 +615,27 @@ public Boo(IServiceScopeFactory scopeFactory)
             {
             }
         }
+
+        private interface IKeyedScopedCollisionService
+        {
+        }
+
+        private class KeyedScopedCollisionSingletonService : IKeyedScopedCollisionService
+        {
+        }
+
+        private class KeyedScopedCollisionScopedService : IKeyedScopedCollisionService
+        {
+            public KeyedScopedCollisionScopedService([FromKeyedServices("Key")] IKeyedScopedCollisionService keyedService)
+            {
+            }
+        }
+
+        private class KeyedScopedCollisionOtherSingleton
+        {
+            public KeyedScopedCollisionOtherSingleton([FromKeyedServices("Key")] IKeyedScopedCollisionService keyedService)
+            {
+            }
+        }
     }
 }