diff --git a/src/coreclr/vm/genmeth.cpp b/src/coreclr/vm/genmeth.cpp
index 62f413ada24e67..300e2873682764 100644
--- a/src/coreclr/vm/genmeth.cpp
+++ b/src/coreclr/vm/genmeth.cpp
@@ -474,10 +474,19 @@ InstantiatedMethodDesc::NewInstantiatedMethodDesc(MethodTable *pExactMT,
             // The canonical instantiation is exempt from constraint checks. It's used as the basis
             // for all other reference instantiations so we can't not load it. The Canon type is
             // not visible to users so it can't be abused.
+            //
+            // Instantiations containing generic variables (type parameters) are also exempt.
+            // Such instantiations arise during JIT access checks when the caller is a generic
+            // method and the EE resolves method specs using the caller's formal type parameters.
+            // Constraint checking for these instantiations would unnecessarily load types
+            // instantiated over the type variables (e.g. IList<TMethod>). The actual constraint
+            // check will happen when the method is instantiated with concrete types.
 
             BOOL fExempt =
                 TypeHandle::IsCanonicalSubtypeInstantiation(methodInst) ||
-                TypeHandle::IsCanonicalSubtypeInstantiation(pNewMD->GetClassInstantiation());
+                TypeHandle::IsCanonicalSubtypeInstantiation(pNewMD->GetClassInstantiation()) ||
+                MethodTable::ComputeContainsGenericVariables(methodInst) ||
+                MethodTable::ComputeContainsGenericVariables(pNewMD->GetClassInstantiation());
 
             if (!fExempt)
             {
diff --git a/src/tests/Loader/classloader/generics/Constraints/Regressions/TypeLoadWithGenericVars/TypeLoadWithGenericVars.cs b/src/tests/Loader/classloader/generics/Constraints/Regressions/TypeLoadWithGenericVars/TypeLoadWithGenericVars.cs
new file mode 100644
index 00000000000000..5084fdc64831d8
--- /dev/null
+++ b/src/tests/Loader/classloader/generics/Constraints/Regressions/TypeLoadWithGenericVars/TypeLoadWithGenericVars.cs
@@ -0,0 +1,50 @@
+// Licensed to the .NET Foundation under one or more agreements.
+// The .NET Foundation licenses this file to you under the MIT license.
+
+// Regression test: access checks triggered by the JIT for generic methods with
+// constraints should not unnecessarily load types instantiated over type variables.
+// The JIT uses the typical method definition (with formal type params) when doing
+// security checks for generic callers, which was triggering constraint checks that
+// loaded types like IList<TMethod> where TMethod is a type variable.
+
+using System;
+using System.Collections.Generic;
+using Xunit;
+
+public class TypeLoadWithGenericVars
+{
+    static void Method<TMethod1_1, TMethod1_2>() where TMethod1_1 : IList<TMethod1_2>
+    {
+        Method2<TMethod1_1, TMethod1_2>();
+    }
+
+    static void Method2<TMethod2_1, TMethod2_2>() where TMethod2_1 : IList<TMethod2_2>
+    {
+    }
+
+    static void MethodA<T1, T2, T3>()
+        where T1 : IList<T2>
+        where T2 : IList<T3>
+    {
+        MethodB<T1, T2, T3>();
+    }
+
+    static void MethodB<U1, U2, U3>()
+        where U1 : IList<U2>
+        where U2 : IList<U3>
+    {
+    }
+
+    [Fact]
+    public static void TestEntryPoint()
+    {
+        // Call with concrete types that satisfy the constraints.
+        // The JIT will use the typical method definition for access checks,
+        // which previously caused unnecessary loading of IList<TMethod1_2>, etc.
+        Method<List<int>, int>();
+        Method<int[], int>();
+
+        // Test with chained generic constraints
+        MethodA<List<List<int>>, List<int>, int>();
+    }
+}
diff --git a/src/tests/Loader/classloader/generics/Constraints/Regressions/TypeLoadWithGenericVars/TypeLoadWithGenericVars.csproj b/src/tests/Loader/classloader/generics/Constraints/Regressions/TypeLoadWithGenericVars/TypeLoadWithGenericVars.csproj
new file mode 100644
index 00000000000000..30d4804022360c
--- /dev/null
+++ b/src/tests/Loader/classloader/generics/Constraints/Regressions/TypeLoadWithGenericVars/TypeLoadWithGenericVars.csproj
@@ -0,0 +1,11 @@
+<Project Sdk="Microsoft.NET.Sdk">
+  <PropertyGroup>
+    <CLRTestPriority>1</CLRTestPriority>
+  </PropertyGroup>
+  <ItemGroup>
+    <Compile Include="$(MSBuildProjectName).cs" />
+  </ItemGroup>
+  <ItemGroup>
+    <ProjectReference Include="$(TestLibraryProjectPath)" />
+  </ItemGroup>
+</Project>