diff --git a/src/libraries/System.IO.FileSystem/src/System/IO/FileSystem.Unix.cs b/src/libraries/System.IO.FileSystem/src/System/IO/FileSystem.Unix.cs
index b91936824fbc36..99f9c98a30e9d5 100644
--- a/src/libraries/System.IO.FileSystem/src/System/IO/FileSystem.Unix.cs
+++ b/src/libraries/System.IO.FileSystem/src/System/IO/FileSystem.Unix.cs
@@ -361,19 +361,6 @@ public static void MoveDirectory(string sourceFullPath, string destFullPath)
                 throw new IOException(SR.Format(SR.IO_AlreadyExists_Name, destFullPath));
             }
 
-#if TARGET_BROWSER
-            // renaming a file doesn't return correct error code on emscripten if one of the parent paths does not exist,
-            // manually workaround it for now (https://github.com/dotnet/runtime/issues/40305)
-            if (!Directory.Exists(Path.GetDirectoryName(sourceFullPath)))
-            {
-                throw new DirectoryNotFoundException(SR.Format(SR.IO_PathNotFound_Path, sourceFullPath));
-            }
-            if (!Directory.Exists(Path.GetDirectoryName(destFullPath)))
-            {
-                throw new DirectoryNotFoundException(SR.Format(SR.IO_PathNotFound_Path, destFullPath));
-            }
-#endif
-
             if (Interop.Sys.Rename(sourceFullPath, destFullPath) < 0)
             {
                 Interop.ErrorInfo errorInfo = Interop.Sys.GetLastErrorInfo();