diff --git a/src/coreclr/nativeaot/System.Private.CoreLib/src/System/Runtime/InteropServices/ComWrappers.NativeAot.cs b/src/coreclr/nativeaot/System.Private.CoreLib/src/System/Runtime/InteropServices/ComWrappers.NativeAot.cs
index 0bb2fb389dd13d..85fa9a7277f5be 100644
--- a/src/coreclr/nativeaot/System.Private.CoreLib/src/System/Runtime/InteropServices/ComWrappers.NativeAot.cs
+++ b/src/coreclr/nativeaot/System.Private.CoreLib/src/System/Runtime/InteropServices/ComWrappers.NativeAot.cs
@@ -409,23 +409,16 @@ static bool IsRootedCallback(IntPtr pObj)
 #pragma warning restore CS8500
             }
 
-            private ManagedObjectWrapper* _wrapper;
-            private object _wrappedObject;
+            private readonly ManagedObjectWrapper* _wrapper;
+            private readonly ManagedObjectWrapperReleaser _releaser;
+            private readonly object _wrappedObject;
 
             public ManagedObjectWrapperHolder(ManagedObjectWrapper* wrapper, object wrappedObject)
             {
                 _wrapper = wrapper;
                 _wrappedObject = wrappedObject;
-            }
-
-            public void InitializeHandle()
-            {
-                IntPtr handle = RuntimeImports.RhHandleAllocRefCounted(this);
-                IntPtr prev = Interlocked.CompareExchange(ref _wrapper->HolderHandle, handle, IntPtr.Zero);
-                if (prev != IntPtr.Zero)
-                {
-                    RuntimeImports.RhHandleFree(handle);
-                }
+                _releaser = new ManagedObjectWrapperReleaser(wrapper);
+                _wrapper->HolderHandle = RuntimeImports.RhHandleAllocRefCounted(this);
             }
 
             public unsafe IntPtr ComIp => _wrapper->As(in ComWrappers.IID_IUnknown);
@@ -433,13 +426,34 @@ public void InitializeHandle()
             public object WrappedObject => _wrappedObject;
 
             public uint AddRef() => _wrapper->AddRef();
+        }
+
+        internal unsafe class ManagedObjectWrapperReleaser
+        {
+            private ManagedObjectWrapper* _wrapper;
+
+            public ManagedObjectWrapperReleaser(ManagedObjectWrapper* wrapper)
+            {
+                _wrapper = wrapper;
+            }
 
-            ~ManagedObjectWrapperHolder()
+            ~ManagedObjectWrapperReleaser()
             {
+                IntPtr refCountedHandle = _wrapper->HolderHandle;
+                if (refCountedHandle != IntPtr.Zero && RuntimeImports.RhHandleGet(refCountedHandle) != null)
+                {
+                    // The ManagedObjectWrapperHolder has not been fully collected, so it is still
+                    // potentially reachable via the Conditional Weak Table.
+                    // Keep ourselves alive in case the wrapped object is resurrected.
+                    GC.ReRegisterForFinalize(this);
+                    return;
+                }
+
                 // Release GC handle created when MOW was built.
                 if (_wrapper->Destroy())
                 {
                     NativeMemory.Free(_wrapper);
+                    _wrapper = null;
                 }
                 else
                 {
@@ -531,7 +545,7 @@ public unsafe IntPtr GetOrCreateComInterfaceForObject(object instance, CreateCom
                 ManagedObjectWrapper* value = CreateCCW(c, flags);
                 return new ManagedObjectWrapperHolder(value, c);
             });
-            ccwValue.InitializeHandle();
+            ccwValue.AddRef();
             return ccwValue.ComIp;
         }
 
@@ -581,7 +595,7 @@ public unsafe IntPtr GetOrCreateComInterfaceForObject(object instance, CreateCom
             }
 
             mow->HolderHandle = IntPtr.Zero;
-            mow->RefCount = 1;
+            mow->RefCount = 0;
             mow->UserDefinedCount = userDefinedCount;
             mow->UserDefined = userDefined;
             mow->Flags = (CreateComInterfaceFlagsEx)flags;
diff --git a/src/tests/Interop/COM/ComWrappers/API/Program.cs b/src/tests/Interop/COM/ComWrappers/API/Program.cs
index 56c2748087e37f..fef40e8d5d0d1d 100644
--- a/src/tests/Interop/COM/ComWrappers/API/Program.cs
+++ b/src/tests/Interop/COM/ComWrappers/API/Program.cs
@@ -319,12 +319,12 @@ unsafe static void CallSetValue(TestComWrappers wrappers, Test testInstance, int
                 Assert.NotEqual(IntPtr.Zero, nativeInstance);
 
                 var iid = typeof(ITest).GUID;
-                IntPtr itestPtr;
+                nint itestPtr;
                 Assert.Equal(0, Marshal.QueryInterface(nativeInstance, ref iid, out itestPtr));
 
-                var inst = Marshal.PtrToStructure<VtblPtr>(itestPtr);
-                var vtbl = Marshal.PtrToStructure<ITestVtbl>(inst.Vtbl);
-                var setValue = (delegate* unmanaged<IntPtr, int, int>)vtbl.SetValue;
+                var inst = (ComWrappers.ComInterfaceDispatch*)itestPtr;
+                var vtbl = (ITestVtbl*)(inst->Vtable);
+                var setValue = (delegate* unmanaged<nint, int, int>)vtbl->SetValue;
 
                 Assert.Equal(0, setValue(itestPtr, value));
                 Assert.Equal(value, testInstance.GetValue());
@@ -332,7 +332,63 @@ unsafe static void CallSetValue(TestComWrappers wrappers, Test testInstance, int
                 // release for QueryInterface
                 Assert.Equal(1, Marshal.Release(itestPtr));
                 // release for GetOrCreateComInterfaceForObject
-                Assert.Equal(0, Marshal.Release(itestPtr));
+                Assert.Equal(0, Marshal.Release(nativeInstance));
+            }
+        }
+
+        [MethodImpl(MethodImplOptions.NoInlining)]
+        [Fact]
+        public void ValidateResurrection()
+        {
+            Console.WriteLine($"Running {nameof(ValidateResurrection)}...");
+
+            var wrappers = new TestComWrappers();
+
+            try
+            {
+                CreateResurrectingTestInstance(wrappers);
+
+                ForceGC();
+
+                CallSetValue(wrappers);
+            }
+            finally
+            {
+                Test.Resurrected = null;
+            }
+
+            [MethodImpl(MethodImplOptions.NoInlining)]
+            static void CreateResurrectingTestInstance(ComWrappers wrapper)
+            {
+                Test testInstance = new Test()
+                {
+                    EnableResurrection = true,
+                };
+                IntPtr nativeInstance = wrapper.GetOrCreateComInterfaceForObject(testInstance, CreateComInterfaceFlags.None);
+                Assert.Equal(0, Marshal.Release(nativeInstance));
+            }
+
+            unsafe static void CallSetValue(ComWrappers wrappers)
+            {
+                Assert.NotEqual(null, Test.Resurrected);
+                IntPtr nativeInstance = wrappers.GetOrCreateComInterfaceForObject(Test.Resurrected, CreateComInterfaceFlags.None);
+                Assert.NotEqual(IntPtr.Zero, nativeInstance);
+
+                var iid = typeof(ITest).GUID;
+                nint itestPtr;
+                Assert.Equal(0, Marshal.QueryInterface(nativeInstance, ref iid, out itestPtr));
+
+                var inst = (ComWrappers.ComInterfaceDispatch*)itestPtr;
+                var vtbl = (ITestVtbl*)(inst->Vtable);
+                var setValue = (delegate* unmanaged<nint, int, int>)vtbl->SetValue;
+
+                Assert.Equal(0, setValue(itestPtr, 42));
+                Assert.Equal(42, Test.Resurrected.GetValue());
+
+                // release for QueryInterface
+                Assert.Equal(1, Marshal.Release(itestPtr));
+                // release for GetOrCreateComInterfaceForObject
+                Assert.Equal(0, Marshal.Release(nativeInstance));
             }
         }
 
diff --git a/src/tests/Interop/COM/ComWrappers/Common.cs b/src/tests/Interop/COM/ComWrappers/Common.cs
index 41fdf348053dba..748c5f9c5c701e 100644
--- a/src/tests/Interop/COM/ComWrappers/Common.cs
+++ b/src/tests/Interop/COM/ComWrappers/Common.cs
@@ -19,17 +19,25 @@ interface ITest
 
     class Test : ITest, ICustomQueryInterface
     {
+        public static Test Resurrected;
         public static int InstanceCount = 0;
 
         private int id;
         private int value = -1;
         public Test() { id = Interlocked.Increment(ref InstanceCount); }
-        ~Test() { Interlocked.Decrement(ref InstanceCount); id = -1; }
+        ~Test()
+        {
+            Interlocked.Decrement(ref InstanceCount);
+            id = -1;
+            if (EnableResurrection)
+                Resurrected = this;
+        }
 
         public void SetValue(int i) => this.value = i;
         public int GetValue() => this.value;
 
         public bool EnableICustomQueryInterface { get; set; } = false;
+        public bool EnableResurrection { get; set; } = false;
         public Guid ICustomQueryInterface_GetInterfaceIID { get; set; }
         public IntPtr ICustomQueryInterface_GetInterfaceResult { get; set; }