diff --git a/common/settings.h b/common/settings.h
index 17408322eedb3..8de173511d4c4 100644
--- a/common/settings.h
+++ b/common/settings.h
@@ -175,6 +175,14 @@ struct Settings {
   // call is made.
   fml::closure root_isolate_shutdown_callback;
   fml::closure isolate_shutdown_callback;
+  // A callback made in the isolate scope of the service isolate when it is
+  // launched. Care must be taken to ensure that callers are assigning callbacks
+  // to the settings object used to launch the VM. If an existing VM is used to
+  // launch an isolate using these settings, the callback will be ignored as the
+  // service isolate has already been launched. Also, this callback will only be
+  // made in the modes in which the service isolate is eligible for launch
+  // (debug and profile).
+  fml::closure service_isolate_create_callback;
   // The callback made on the UI thread in an isolate scope when the engine
   // detects that the framework is idle. The VM also uses this time to perform
   // tasks suitable when idling. Due to this, embedders are still advised to be
diff --git a/runtime/dart_isolate.cc b/runtime/dart_isolate.cc
index 2fa50132e6750..ae655f00e07cf 100644
--- a/runtime/dart_isolate.cc
+++ b/runtime/dart_isolate.cc
@@ -698,6 +698,14 @@ Dart_Isolate DartIsolate::DartCreateAndStartServiceIsolate(
 
   flags->load_vmservice_library = true;
 
+#if (FLUTTER_RUNTIME_MODE != FLUTTER_RUNTIME_MODE_DEBUG)
+  // TODO(68663): The service isolate in debug mode is always launched without
+  // sound null safety. Fix after the isolate snapshot data is created with the
+  // right flags.
+  flags->null_safety =
+      vm_data->GetIsolateSnapshot()->IsNullSafetyEnabled(nullptr);
+#endif
+
   std::weak_ptr<DartIsolate> weak_service_isolate =
       DartIsolate::CreateRootIsolate(
           vm_data->GetSettings(),         // settings
@@ -739,6 +747,10 @@ Dart_Isolate DartIsolate::DartCreateAndStartServiceIsolate(
     return nullptr;
   }
 
+  if (auto callback = vm_data->GetSettings().service_isolate_create_callback) {
+    callback();
+  }
+
   if (auto service_protocol = DartVMRef::GetServiceProtocol()) {
     service_protocol->ToggleHooks(true);
   } else {
diff --git a/runtime/dart_isolate_unittests.cc b/runtime/dart_isolate_unittests.cc
index c95235025ee8e..bfdbfa9914669 100644
--- a/runtime/dart_isolate_unittests.cc
+++ b/runtime/dart_isolate_unittests.cc
@@ -321,5 +321,59 @@ TEST_F(DartIsolateTest, CanRecieveArguments) {
   Wait();
 }
 
+TEST_F(DartIsolateTest, CanCreateServiceIsolate) {
+#if (FLUTTER_RUNTIME_MODE != FLUTTER_RUNTIME_MODE_DEBUG) && \
+    (FLUTTER_RUNTIME_MODE != FLUTTER_RUNTIME_MODE_PROFILE)
+  GTEST_SKIP();
+#endif
+  ASSERT_FALSE(DartVMRef::IsInstanceRunning());
+  fml::AutoResetWaitableEvent service_isolate_latch;
+  auto settings = CreateSettingsForFixture();
+  settings.enable_observatory = true;
+  settings.observatory_port = 0;
+  settings.observatory_host = "127.0.0.1";
+  settings.enable_service_port_fallback = true;
+  settings.service_isolate_create_callback = [&service_isolate_latch]() {
+    service_isolate_latch.Signal();
+  };
+  auto vm_ref = DartVMRef::Create(settings);
+  ASSERT_TRUE(vm_ref);
+  auto vm_data = vm_ref.GetVMData();
+  ASSERT_TRUE(vm_data);
+  TaskRunners task_runners(GetCurrentTestName(),    //
+                           GetCurrentTaskRunner(),  //
+                           GetCurrentTaskRunner(),  //
+                           GetCurrentTaskRunner(),  //
+                           GetCurrentTaskRunner()   //
+  );
+
+  auto isolate_configuration =
+      IsolateConfiguration::InferFromSettings(settings);
+  auto weak_isolate = DartIsolate::CreateRunningRootIsolate(
+      vm_data->GetSettings(),              // settings
+      vm_data->GetIsolateSnapshot(),       // isolate snapshot
+      std::move(task_runners),             // task runners
+      nullptr,                             // window
+      {},                                  // snapshot delegate
+      {},                                  // hint freed delegate
+      {},                                  // io manager
+      {},                                  // unref queue
+      {},                                  // image decoder
+      "main.dart",                         // advisory uri
+      "main",                              // advisory entrypoint,
+      DartIsolate::Flags{},                // flags
+      settings.isolate_create_callback,    // isolate create callback
+      settings.isolate_shutdown_callback,  // isolate shutdown callback
+      "main",                              // dart entrypoint
+      std::nullopt,                        // dart entrypoint library
+      std::move(isolate_configuration)     // isolate configuration
+  );
+  auto root_isolate = weak_isolate.lock();
+  ASSERT_TRUE(root_isolate);
+  ASSERT_EQ(root_isolate->GetPhase(), DartIsolate::Phase::Running);
+  service_isolate_latch.Wait();
+  ASSERT_TRUE(root_isolate->Shutdown());
+}
+
 }  // namespace testing
 }  // namespace flutter