diff --git a/.release-please-manifest.json b/.release-please-manifest.json
index 00d392a248..62c031f3f8 100644
--- a/.release-please-manifest.json
+++ b/.release-please-manifest.json
@@ -1,3 +1,3 @@
 {
-    ".": "3.53.0"
+    ".": "3.54.0"
 }
diff --git a/CHANGELOG.md b/CHANGELOG.md
index 0bde684970..ee56542822 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -4,6 +4,21 @@
 
 [1]: https://pypi.org/project/google-cloud-spanner/#history
 
+## [3.54.0](https://github.com/googleapis/python-spanner/compare/v3.53.0...v3.54.0) (2025-04-28)
+
+
+### Features
+
+* Add interval type support ([#1340](https://github.com/googleapis/python-spanner/issues/1340)) ([6ca9b43](https://github.com/googleapis/python-spanner/commit/6ca9b43c3038eca1317c7c9b7e3543b5f1bc68ad))
+* Add sample for pre-split feature ([#1333](https://github.com/googleapis/python-spanner/issues/1333)) ([ca76108](https://github.com/googleapis/python-spanner/commit/ca76108809174e4f3eea38d7ac2463d9b4c73304))
+* Add SQL statement for begin transaction isolation level ([#1331](https://github.com/googleapis/python-spanner/issues/1331)) ([3ac0f91](https://github.com/googleapis/python-spanner/commit/3ac0f9131b38e5cfb2b574d3d73b03736b871712))
+* Support transaction isolation level in dbapi ([#1327](https://github.com/googleapis/python-spanner/issues/1327)) ([03400c4](https://github.com/googleapis/python-spanner/commit/03400c40f1c1cc73e51733f2a28910a8dd78e7d9))
+
+
+### Bug Fixes
+
+* Improve client-side regex statement parser ([#1328](https://github.com/googleapis/python-spanner/issues/1328)) ([b3c259d](https://github.com/googleapis/python-spanner/commit/b3c259deec817812fd8e4940faacf4a927d0d69c))
+
 ## [3.53.0](https://github.com/googleapis/python-spanner/compare/v3.52.0...v3.53.0) (2025-03-12)
 
 
diff --git a/google/cloud/spanner_admin_database_v1/gapic_version.py b/google/cloud/spanner_admin_database_v1/gapic_version.py
index 9b205942db..9f7e08d550 100644
--- a/google/cloud/spanner_admin_database_v1/gapic_version.py
+++ b/google/cloud/spanner_admin_database_v1/gapic_version.py
@@ -13,4 +13,4 @@
 # See the License for the specific language governing permissions and
 # limitations under the License.
 #
-__version__ = "3.53.0"  # {x-release-please-version}
+__version__ = "3.54.0"  # {x-release-please-version}
diff --git a/google/cloud/spanner_admin_instance_v1/gapic_version.py b/google/cloud/spanner_admin_instance_v1/gapic_version.py
index 9b205942db..9f7e08d550 100644
--- a/google/cloud/spanner_admin_instance_v1/gapic_version.py
+++ b/google/cloud/spanner_admin_instance_v1/gapic_version.py
@@ -13,4 +13,4 @@
 # See the License for the specific language governing permissions and
 # limitations under the License.
 #
-__version__ = "3.53.0"  # {x-release-please-version}
+__version__ = "3.54.0"  # {x-release-please-version}
diff --git a/google/cloud/spanner_v1/gapic_version.py b/google/cloud/spanner_v1/gapic_version.py
index 9b205942db..9f7e08d550 100644
--- a/google/cloud/spanner_v1/gapic_version.py
+++ b/google/cloud/spanner_v1/gapic_version.py
@@ -13,4 +13,4 @@
 # See the License for the specific language governing permissions and
 # limitations under the License.
 #
-__version__ = "3.53.0"  # {x-release-please-version}
+__version__ = "3.54.0"  # {x-release-please-version}
diff --git a/samples/generated_samples/snippet_metadata_google.spanner.admin.database.v1.json b/samples/generated_samples/snippet_metadata_google.spanner.admin.database.v1.json
index fc77bc1740..9bbabdab00 100644
--- a/samples/generated_samples/snippet_metadata_google.spanner.admin.database.v1.json
+++ b/samples/generated_samples/snippet_metadata_google.spanner.admin.database.v1.json
@@ -8,7 +8,7 @@
     ],
     "language": "PYTHON",
     "name": "google-cloud-spanner-admin-database",
-    "version": "3.53.0"
+    "version": "3.54.0"
   },
   "snippets": [
     {
diff --git a/samples/generated_samples/snippet_metadata_google.spanner.admin.instance.v1.json b/samples/generated_samples/snippet_metadata_google.spanner.admin.instance.v1.json
index 74eaaff2f8..765c9d46ed 100644
--- a/samples/generated_samples/snippet_metadata_google.spanner.admin.instance.v1.json
+++ b/samples/generated_samples/snippet_metadata_google.spanner.admin.instance.v1.json
@@ -8,7 +8,7 @@
     ],
     "language": "PYTHON",
     "name": "google-cloud-spanner-admin-instance",
-    "version": "3.53.0"
+    "version": "3.54.0"
   },
   "snippets": [
     {
diff --git a/samples/generated_samples/snippet_metadata_google.spanner.v1.json b/samples/generated_samples/snippet_metadata_google.spanner.v1.json
index ba20d6b76a..c9c643d8b2 100644
--- a/samples/generated_samples/snippet_metadata_google.spanner.v1.json
+++ b/samples/generated_samples/snippet_metadata_google.spanner.v1.json
@@ -8,7 +8,7 @@
     ],
     "language": "PYTHON",
     "name": "google-cloud-spanner",
-    "version": "3.53.0"
+    "version": "3.54.0"
   },
   "snippets": [
     {