diff --git a/.pre-commit-config.yaml b/.pre-commit-config.yaml
index 16307ab80d29..22cb07869546 100644
--- a/.pre-commit-config.yaml
+++ b/.pre-commit-config.yaml
@@ -14,11 +14,6 @@ repos:
     hooks:
       - id: black
         language_version: python3.10
-  - repo: https://github.com/pycqa/isort
-    rev: 5.12.0 # must match requirements-tests.txt
-    hooks:
-      - id: isort
-        name: isort (python)
   - repo: https://github.com/astral-sh/ruff-pre-commit
     rev: v0.1.0 # must match requirements-tests.txt and tests.yml
     hooks:
diff --git a/.vscode/extensions.json b/.vscode/extensions.json
index 7200f91a1b4c..01b9ad4369db 100644
--- a/.vscode/extensions.json
+++ b/.vscode/extensions.json
@@ -7,7 +7,6 @@
         "editorconfig.editorconfig",
         "ms-python.black-formatter",
         "ms-python.flake8",
-        "ms-python.isort",
         "ms-python.mypy-type-checker",
         "ms-python.python",
         "ms-python.vscode-pylance",
@@ -16,17 +15,19 @@
     ],
     "unwantedRecommendations": [
         /*
-         * Don't recommend by default for this workspace
+        * Don't recommend by default for this workspace
         */
         "christian-kohler.npm-intellisense",
         /*
-         * Must disable in this workspace
-         * https://github.com/microsoft/vscode/issues/40239
+        * Must disable in this workspace
+        * https://github.com/microsoft/vscode/issues/40239
         */
         // even-better-toml has format on save
         "bungcip.better-toml",
         // Don't use two mypy extensions simultaneously
         "matangover.mypy",
+        // Use Ruff instead
+        "ms-python.isort",
         // We use Black
         "ms-python.autopep8",
         // Not using pylint
diff --git a/.vscode/settings.default.json b/.vscode/settings.default.json
index 6f0b0630e968..d06ef6034aed 100644
--- a/.vscode/settings.default.json
+++ b/.vscode/settings.default.json
@@ -80,7 +80,6 @@
     "python.linting.prospectorEnabled": false,
     "python.linting.pylamaEnabled": false,
     "python.linting.pylintEnabled": false,
-    // Not using bandit
     "python.linting.banditEnabled": false,
     // python.analysis is Pylance (pyright) configurations
     "python.analysis.fixAll": [
@@ -109,13 +108,12 @@
         "--config=.flake8"
     ],
     "flake8.importStrategy": "fromEnvironment",
-    "isort.check": true,
-    "isort.importStrategy": "fromEnvironment",
     "black-formatter.importStrategy": "fromEnvironment",
+    // Using Ruff instead of isort
+    "isort.check": false,
     "ruff.importStrategy": "fromEnvironment",
     "ruff.fixAll": true,
-    // Conflict between Ruff and isort
-    "ruff.organizeImports": false,
+    "ruff.organizeImports": true,
     "evenBetterToml.formatter.alignComments": false,
     "evenBetterToml.formatter.alignEntries": false,
     "evenBetterToml.formatter.allowedBlankLines": 1,
diff --git a/CONTRIBUTING.md b/CONTRIBUTING.md
index c9a4bd71fa1a..9390c9d9dc60 100644
--- a/CONTRIBUTING.md
+++ b/CONTRIBUTING.md
@@ -30,8 +30,7 @@ Typeshed runs continuous integration (CI) on all pull requests. This means that
 if you file a pull request (PR), our full test suite
 -- including our linter, [Flake8](https://github.com/PyCQA/flake8) --
 is run on your PR. It also means that bots will automatically apply
-changes to your PR (using [Black](https://github.com/psf/black),
-[isort](https://github.com/PyCQA/isort) and
+changes to your PR (using [Black](https://github.com/psf/black) and
 [Ruff](https://github.com/astral-sh/ruff)) to fix any formatting issues.
 This frees you up to ignore all local setup on your side, focus on the
 code and rely on the CI to fix everything, or point you to the places that
@@ -87,8 +86,7 @@ terminal to install all non-pytype requirements:
 
 ## Code formatting
 
-The code is formatted using [`Black`](https://github.com/psf/black)
-and [`isort`](https://github.com/PyCQA/isort).
+The code is formatted using [`Black`](https://github.com/psf/black).
 Various other autofixes are
 also performed by [`Ruff`](https://github.com/astral-sh/ruff).
 
@@ -101,8 +99,7 @@ That being said, if you *want* to run the checks locally when you commit,
 you're free to do so. Either run the following manually...
 
 ```bash
-(.venv)$ isort .
-(.venv)$ ruff .
+(.venv)$ ruff check .
 (.venv)$ black .
 ```
 
diff --git a/pyproject.toml b/pyproject.toml
index 6a7ae3e93db9..250fd3d91b57 100644
--- a/pyproject.toml
+++ b/pyproject.toml
@@ -7,15 +7,13 @@ skip_magic_trailing_comma = true
 # for just these files, but doesn't seem possible yet.
 force-exclude = ".*_pb2.pyi"
 
-[tool.isort]
-profile = "black"
-combine_as_imports = true
-line_length = 130
-skip = [".git", ".github", ".venv"]
-extra_standard_library = [
+[tool.ruff.isort]
+split-on-trailing-comma = false
+combine-as-imports = true
+extra-standard-library = [
     "_typeshed",
     "typing_extensions",
-    # Extra modules not recognized by isort
+    # Extra modules not recognized by Ruff/isort
     "_ast",
     "_bisect",
     "_bootlocale",
@@ -56,7 +54,7 @@ extra_standard_library = [
     "opcode",
     "pyexpat",
 ]
-known_first_party = ["parse_metadata", "utils"]
+known-first-party = ["parse_metadata", "utils"]
 
 [tool.ruff]
 line-length = 130
@@ -77,12 +75,11 @@ exclude = [
     ".venv",
     "env",
 ]
-
-# Only enable rules that have safe autofixes;
-# only enable rules that are relevant to stubs
 select = [
-    "F401", # Remove unused imports
     "FA", # flake8-future-annotations
+    "I", # isort
+    # Only enable rules that have safe autofixes:
+    "F401", # Remove unused imports
     "PYI009", # use `...`, not `pass`, in empty class bodies
     "PYI010", # function bodies must be empty
     "PYI012", # class bodies must not contain `pass`
diff --git a/requirements-tests.txt b/requirements-tests.txt
index 9201a41d6cce..35a9f80f761b 100644
--- a/requirements-tests.txt
+++ b/requirements-tests.txt
@@ -6,7 +6,6 @@ flake8==6.1.0            # must match .pre-commit-config.yaml
 flake8-bugbear==23.9.16  # must match .pre-commit-config.yaml
 flake8-noqa==1.3.2       # must match .pre-commit-config.yaml
 flake8-pyi==23.10.0      # must match .pre-commit-config.yaml
-isort==5.12.0            # must match .pre-commit-config.yaml
 mypy==1.6.1
 pre-commit-hooks==4.5.0  # must match .pre-commit-config.yaml
 pytype==2023.10.17; platform_system != "Windows" and python_version < "3.12"
diff --git a/scripts/create_baseline_stubs.py b/scripts/create_baseline_stubs.py
index 2978d40f9de1..7fb8db00e6b3 100755
--- a/scripts/create_baseline_stubs.py
+++ b/scripts/create_baseline_stubs.py
@@ -65,14 +65,9 @@ def run_black(stub_dir: str) -> None:
     subprocess.run(["black", stub_dir])
 
 
-def run_isort(stub_dir: str) -> None:
-    print(f"Running isort: isort {stub_dir}")
-    subprocess.run([sys.executable, "-m", "isort", stub_dir])
-
-
 def run_ruff(stub_dir: str) -> None:
-    print(f"Running Ruff: ruff {stub_dir}")
-    subprocess.run([sys.executable, "-m", "ruff", stub_dir])
+    print(f"Running Ruff: ruff check {stub_dir} --fix-only")
+    subprocess.run([sys.executable, "-m", "ruff", "check", stub_dir, "--fix-only"])
 
 
 async def get_project_urls_from_pypi(project: str, session: aiohttp.ClientSession) -> dict[str, str]:
@@ -189,7 +184,7 @@ def add_pyright_exclusion(stub_dir: str) -> None:
 def main() -> None:
     parser = argparse.ArgumentParser(
         description="""Generate baseline stubs automatically for an installed pip package
-                       using stubgen. Also run Black, isort and Ruff. If the name of
+                       using stubgen. Also run Black and Ruff. If the name of
                        the project is different from the runtime Python package name, you may
                        need to use --package (example: --package yaml PyYAML)."""
     )
@@ -239,7 +234,6 @@ def main() -> None:
     run_stubdefaulter(stub_dir)
 
     run_ruff(stub_dir)
-    run_isort(stub_dir)
     run_black(stub_dir)
 
     create_metadata(project, stub_dir, version)
diff --git a/scripts/generate_proto_stubs.sh b/scripts/generate_proto_stubs.sh
index 4f8f65c2f748..011b1e8d0c7a 100755
--- a/scripts/generate_proto_stubs.sh
+++ b/scripts/generate_proto_stubs.sh
@@ -45,7 +45,7 @@ PYTHON_PROTOBUF_DIR="protobuf-$PYTHON_PROTOBUF_VERSION"
 VENV=venv
 python3 -m venv "$VENV"
 source "$VENV/bin/activate"
-pip install -r "$REPO_ROOT/requirements-tests.txt"  # for Black and isort
+pip install -r "$REPO_ROOT/requirements-tests.txt"  # for Black and Ruff
 
 # Install mypy-protobuf
 pip install mypy-protobuf=="$MYPY_PROTOBUF_VERSION"
@@ -73,7 +73,7 @@ PROTO_FILES=$(grep "GenProto.*google" $PYTHON_PROTOBUF_DIR/python/setup.py | \
 # shellcheck disable=SC2086
 protoc_install/bin/protoc --proto_path="$PYTHON_PROTOBUF_DIR/src" --mypy_out="relax_strict_optional_primitives:$REPO_ROOT/stubs/protobuf" $PROTO_FILES
 
-isort "$REPO_ROOT/stubs/protobuf"
+ruff check "$REPO_ROOT/stubs/protobuf" --fix-only
 black "$REPO_ROOT/stubs/protobuf"
 
 sed --in-place="" \
diff --git a/scripts/runtests.py b/scripts/runtests.py
index 5409fe31fab2..bec0e47ccaa0 100644
--- a/scripts/runtests.py
+++ b/scripts/runtests.py
@@ -83,9 +83,7 @@ def main() -> None:
 
     # Run formatters first. Order matters.
     print("\nRunning Ruff...")
-    subprocess.run([sys.executable, "-m", "ruff", path])
-    print("\nRunning isort...")
-    subprocess.run([sys.executable, "-m", "isort", path])
+    subprocess.run([sys.executable, "-m", "ruff", "check", path])
     print("\nRunning Black...")
     black_result = subprocess.run([sys.executable, "-m", "black", path])
     if black_result.returncode == 123:
diff --git a/scripts/sync_tensorflow_protobuf_stubs.sh b/scripts/sync_tensorflow_protobuf_stubs.sh
index 1626338febe0..11471a86c2aa 100755
--- a/scripts/sync_tensorflow_protobuf_stubs.sh
+++ b/scripts/sync_tensorflow_protobuf_stubs.sh
@@ -61,7 +61,7 @@ rm tensorflow/compiler/xla/service/hlo_execution_profile_data_pb2.pyi \
    tensorflow/core/protobuf/worker_service_pb2.pyi \
    tensorflow/core/util/example_proto_fast_parsing_test_pb2.pyi
 
-isort "$REPO_ROOT/stubs/tensorflow/tensorflow"
+ruff check "$REPO_ROOT/stubs/tensorflow/tensorflow" --fix-only
 black "$REPO_ROOT/stubs/tensorflow/tensorflow"
 
 sed --in-place="" \
diff --git a/stdlib/csv.pyi b/stdlib/csv.pyi
index 53425fbcccb1..f48d9d2ff263 100644
--- a/stdlib/csv.pyi
+++ b/stdlib/csv.pyi
@@ -23,7 +23,7 @@ from _csv import (
 )
 
 if sys.version_info >= (3, 12):
-    from _csv import QUOTE_STRINGS as QUOTE_STRINGS, QUOTE_NOTNULL as QUOTE_NOTNULL
+    from _csv import QUOTE_NOTNULL as QUOTE_NOTNULL, QUOTE_STRINGS as QUOTE_STRINGS
 from _typeshed import SupportsWrite
 from collections.abc import Collection, Iterable, Iterator, Mapping, Sequence
 from typing import Any, Generic, TypeVar, overload
diff --git a/tests/check_consistent.py b/tests/check_consistent.py
index 91fdf68bdbb6..1fc40db576c9 100755
--- a/tests/check_consistent.py
+++ b/tests/check_consistent.py
@@ -23,7 +23,7 @@
 
 # These type checkers and linters must have exact versions in the requirements file to ensure
 # consistent CI runs.
-linters = {"black", "flake8", "flake8-bugbear", "flake8-noqa", "flake8-pyi", "isort", "ruff", "mypy", "pytype"}
+linters = {"black", "flake8", "flake8-bugbear", "flake8-noqa", "flake8-pyi", "ruff", "mypy", "pytype"}
 
 
 def assert_consistent_filetypes(