diff --git a/CHANGELOG.md b/CHANGELOG.md
index 86b217a..21bd794 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -26,6 +26,7 @@ Types of changes:
 ### Removed
 
 ### Fixed
+- Fixed `consolidate_qubit_registers` mutating AST nodes in-place, causing a `ValidationError` when `unroll(consolidate_qubits=True)` is called more than once on the same `QasmModule`. ([#297](https://github.com/qBraid/pyqasm/pull/297))
 - Fixed barrier unrolling to preserve multi-qubit barrier statements instead of splitting into individual per-qubit barriers. ([#295](https://github.com/qBraid/pyqasm/pull/295))
 - Added support for physical qubit identifiers (`$0`, `$1`, …) in plain QASM 3 programs, including gates, barriers, measurements, and duplicate-qubit detection. ([#291](https://github.com/qBraid/pyqasm/pull/291))
 - Updated CI to use `macos-15-intel` image due to deprecation of `macos-13` image. ([#283](https://github.com/qBraid/pyqasm/pull/283))
diff --git a/src/pyqasm/transformer.py b/src/pyqasm/transformer.py
index 6f0fe2a..8ab2916 100644
--- a/src/pyqasm/transformer.py
+++ b/src/pyqasm/transformer.py
@@ -469,6 +469,10 @@ def consolidate_qubit_registers(  # pylint: disable=too-many-branches, too-many-
         if device_qubits is None:
             device_qubits = sum(global_qreg_size_map.values())
 
+        # Deep-copy so that in-place mutations below never corrupt the
+        # original AST nodes (which may be re-visited on a subsequent unroll).
+        unrolled_stmts = deepcopy(unrolled_stmts)
+
         def _get_pyqasm_device_qubit_index(
             reg: str, idx: int, qubit_reg_offsets: dict[str, int], global_qreg: dict[str, int]
         ):
diff --git a/tests/qasm3/test_device_qubits.py b/tests/qasm3/test_device_qubits.py
index d3c0eec..1c08271 100644
--- a/tests/qasm3/test_device_qubits.py
+++ b/tests/qasm3/test_device_qubits.py
@@ -223,6 +223,34 @@ def test_gates():
     check_unrolled_qasm(dumps(result), expected_qasm)
 
 
+def test_double_unroll_with_consolidate_qubits():
+    """Test that calling unroll(consolidate_qubits=True) twice on the same
+    module does not raise due to in-place AST mutation from the first call."""
+    qasm = """OPENQASM 3.0;
+    include "stdgates.inc";
+    qubit[2] q;
+    qreg q2[3];
+    barrier q2;
+    barrier q, q2;
+    """
+    expected_qasm = """OPENQASM 3.0;
+    qubit[5] __PYQASM_QUBITS__;
+    include "stdgates.inc";
+    barrier __PYQASM_QUBITS__[2], __PYQASM_QUBITS__[3], __PYQASM_QUBITS__[4];
+    barrier __PYQASM_QUBITS__[0], __PYQASM_QUBITS__[1], __PYQASM_QUBITS__[2], __PYQASM_QUBITS__[3], __PYQASM_QUBITS__[4];
+    """
+    mod = loads(qasm)
+    mod.unroll(consolidate_qubits=True)
+    first_result = dumps(mod)
+
+    # Second unroll should produce identical output without raising
+    mod.unroll(consolidate_qubits=True)
+    second_result = dumps(mod)
+
+    check_unrolled_qasm(first_result, expected_qasm)
+    check_unrolled_qasm(second_result, expected_qasm)
+
+
 def test_validate(caplog):
     with pytest.raises(ValidationError, match=r"Total qubits '4' exceed device qubits '3'."):
         with caplog.at_level("ERROR"):