diff --git a/dvc/ignore.py b/dvc/ignore.py
index 7ef67763bd..53436e226f 100644
--- a/dvc/ignore.py
+++ b/dvc/ignore.py
@@ -1,12 +1,15 @@
 import logging
 import os
+import re
+from itertools import groupby
 
 from funcy import cached_property
-from pathspec import PathSpec
 from pathspec.patterns import GitWildMatchPattern
+from pathspec.util import normalize_file
 
 from dvc.path_info import PathInfo
 from dvc.scm.tree import BaseTree
+from dvc.system import System
 from dvc.utils import relpath
 
 logger = logging.getLogger(__name__)
@@ -27,7 +30,16 @@ def __init__(self, ignore_file_path, tree):
         self.dirname = os.path.normpath(os.path.dirname(ignore_file_path))
 
         with tree.open(ignore_file_path, encoding="utf-8") as fobj:
-            self.ignore_spec = PathSpec.from_lines(GitWildMatchPattern, fobj)
+            path_spec_lines = fobj.readlines()
+            regex_pattern_list = map(
+                GitWildMatchPattern.pattern_to_regex, path_spec_lines
+            )
+            self.ignore_spec = [
+                (ignore, re.compile("|".join(item[0] for item in group)))
+                for ignore, group in groupby(
+                    regex_pattern_list, lambda x: x[1]
+                )
+            ]
 
     def __call__(self, root, dirs, files):
         files = [f for f in files if not self.matches(root, f)]
@@ -48,7 +60,16 @@ def matches(self, dirname, basename):
         else:
             return False
 
-        return self.ignore_spec.match_file(path)
+        if not System.is_unix():
+            path = normalize_file(path)
+        return self.ignore(path)
+
+    def ignore(self, path):
+        result = False
+        for ignore, pattern in self.ignore_spec:
+            if pattern.match(path):
+                result = ignore
+        return result
 
     def __hash__(self):
         return hash(self.ignore_file_path)
diff --git a/tests/unit/test_ignore.py b/tests/unit/test_ignore.py
index 1f7c4d8fc3..40f936d091 100644
--- a/tests/unit/test_ignore.py
+++ b/tests/unit/test_ignore.py
@@ -99,3 +99,18 @@ def test_should_ignore_dir(omit_dir):
     new_dirs, _ = ignore(root, dirs, files)
 
     assert set(new_dirs) == {"dir1", "dir2"}
+
+
+def test_ignore_order():
+    dvcignore_path = os.path.join(os.path.sep, "ignore_order", ".dvcignore")
+
+    patterns = ["!ac*", "a*", "!ab*"]
+
+    root = os.path.dirname(dvcignore_path)
+    dirs = ["ignore_order"]
+    files = ["ac", "ab", "aa"]
+
+    ignore = mock_dvcignore(dvcignore_path, patterns)
+    _, new_files = ignore(root, dirs, files)
+
+    assert {"ab"} == set(new_files)