We have been hoping to use VALIS to align large image stacks of up to 400 images, but have been running into issues. Specifically, the program will self-terminate. We would appreciate any advice you could lend.
Currently, we believe the problem resides in the rigid transformation step as the output mask and early steps appear correct. When we divide the data set into smaller stacks, the program was able to align these smaller subgroups individually but self-terminates when asked to then align the subgroup stacks with each other. We did attempt to reduce the pixels per image in an attempt to smooth the process, but it had no effect.
We have been hoping to use VALIS to align large image stacks of up to 400 images, but have been running into issues. Specifically, the program will self-terminate. We would appreciate any advice you could lend.
Currently, we believe the problem resides in the rigid transformation step as the output mask and early steps appear correct. When we divide the data set into smaller stacks, the program was able to align these smaller subgroups individually but self-terminates when asked to then align the subgroup stacks with each other. We did attempt to reduce the pixels per image in an attempt to smooth the process, but it had no effect.