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Description
Hello,
I am tryig to follow your instructions for Making host genome index for bmtagger
Your suggestion dont have threads option
srprism mkindex -i hg38.fa -o hg38.srprism -M 100000
Could you be so kind to add it as an option,
I have tried --threads, --thread, -t, -T - it's says unrecognised argument
I can't use it without specifing the number of threads
And I can't find the solution in google...
checking the options didn't helped also, Is it working on a single thread?
srprism mkindex --help
Fast Short Read Aligner
version 2.4.24-alpha
USAGE:
srprism cmd [--trace-level <min_level>] [--log-file <file-name>] [--input <input-file(s)>] [--input-list <file-name>] [--alt-loc <file-name>] [--output <output-stream>] [--input-format <format-name>] [--input-compression <compression-type>] [--output-format <format-name>] [--memory <megabytes>] [--seg-letters <bases>] [--al-extend <bases>]
COMMON PARAMETERS:
cmd [required]
Action to perform. Possible values are:
* help - get usage help;
* search - search for occurrences of the queries in
the database;
* mkindex - create index from a source database.
--trace-level <min_level> [default: warning]
Minimum message level to report to the log stream. Possible
values are "debug", "info", "warning", "error", "quiet".
--log-file <file-name> [optional]
File for storing diagnostic messages. Default is standard
error.
MKINDEX PARAMETERS:
--input|-i <input-file(s)> [optional]
Source database (may be a comma-separated list of file names).
This options takes precedence over --input-list.
--input-list|-l <file-name> [optional]
Name of the file containing a list of input file names, one
name per line.
--alt-loc|-a <file-name> [optional]
Name of the alternative loci specification file.
--output|-o <output-stream> [required]
Base name for generated database index files.
--input-format|-F <format-name> [default: fasta]
The input format name. The possible values are "fasta",
"fastq", "cfasta", "cfastq".
--input-compression <compression-type> [default: auto]
Compression type used for input. The possible values are
"auto" (default), "none", "gzip", and "bzip2". If the value
given is "auto" then the type of compression is guessed from
the file extension.
--output-format|-O <format-name> [default: standard]
The output format name. The possible values are "standard".
--memory|-M <megabytes> [default: 2048]
Do not use more than this many megabytes of memory for
internal dynamic data structures. This number does not include
the footprint of the executable code, static data, or stack.
--seg-letters <bases> [default: 8192]
Number of letters in sequence store segment. It is recommended
that this value is set to less than half of length of a
typical sequence in the reference database. Each reference
sequence occupies at least one segment and the sequence store
can store at most 2^32 - 1 bases. If the reference has a large
number of very short sequence, decreasing this value can help
to pack more sequences into the sequence store and optimize
memory usage.
--al-extend <bases> [default: 2000]
Number of reference bases by which an alternate locus is
extended to the left (right) in the case of non-fuzzy left
(right) end.
options_parser.cpp:239 [2] key error (unknown key help)
ERROR: options_parser.cpp:239 [2] key error (unknown key help) srprism.cpp:850
(metawrap-env)
I would appreciate any help,
Thanks
Best,
Alla