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A meta-listing of software, training resources, and more from the Cimini Lab at the Broad Institute Imaging Platform

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Cimini Lab resources

Below are ways to collaborate our lab, pointers to our software and its documentation, and the educational resources and papers we have been a part of. We hope these resources help you out!

This page last updated 2025-October-17

Jump to:

About us | Work with us | Software | Educational materials | Papers

About us {#about}

Lab website

Lab members

Lab paper page

Current lab preprints (from Beth's CV)

Beth's Broad bio page with contact email

Beth's CV

Work with us {#collaborate}

Learn about our collaboration models

Image Analysis Collaboration: A Practical Guide

Office hours link (free for anyone! just show up!)

CellProfiler annual support plan

Tool contributions you could make (from Google Summer of Code 2025 proposal)

Attend our semi-annual bootcamp

Software we make {#software}

CellProfiler CellProfiler Analyst Piximi Distributed Something Bilayers
CellProfiler CellProfiler Analyst Piximi Distributed Something Bilayers

CellProfiler {#cellprofiler}

CellProfiler icon

Open source image analysis software. Highly flexible workflow management tool with a robust plugin system.

| Download CellProfiler | CellProfiler Manual | CellProfiler on GitHub | CellProfiler Plugins Documentation | CellProfiler Plugins on Github |

Learn to Use CellProfiler

| CellProfiler Tutorials | CellProfiler Video Tutorials | CellProfiler Examples | CellProfiler Help Forum | CellProfiler pipeline suggester |

Papers about CellProfiler

CellProfiler-plugins, 2023
CellProfiler 4, 2021
CellProfiler 3, 2018

CellProfiler Analyst {#cpa}

CellProfiler Analyst icon

Open source data exploration software.

| Download CellProfiler Analyst | CellProfiler Analyst Manual | CellProfiler Analyst on GitHub| CellProfiler Analyst Help Forum |

Papers about CellProfiler Analyst

CellProfiler Analyst 3, 2021

Piximi {#piximi}

Piximi icon

Open-source, browser-based platform for interactive bioimage analysis. User-friendly interface for annotation, segmentation, classification, and quantitative measurement of microscopy images.

| Piximi.app | Piximi Manual and Tutorials | Piximi Video Tutorials | Piximi on GitHub | Piximi Help Forum |

Papers about Piximi

Preprint, 2024

Distributed-Something {#ds}

Distributed-Something icon

Run almost any encapsulated docker container in the Amazon Web Services infrastructure.

| Distributed-Someting on Github | Distributed-Something Documentation |

Distributed Tools

| Distributed-CellProfiler | Distributed-Collate | Distributed-DeepProfiler | Distributed-Fiji | Distributed-HelloWorld | Distributed-OMEZarrCreator |

Papers about Distributed-Something

Distributed-Something: scripts to leverage AWS storage and computing for distributed workflows at scale, 2023

Bilayers {#bilayers}

Bilayers icon

Open-source specification designed to characterize software containers in terms of expected inputs, outputs, and tunable parameters. Its primary aim is to make bioimage analysis deep-learning algorithms more accessible by automatically generating intuitive, no-terminal-required user interfaces.

| Bilayers documentation | Bilayers on GitHub | Bilayers on Dockerhub | Slide deck describing idea of Bilayers | Bilayers video tutorial | Old demo application of Bilayers |

Educational materials {#educational}

Jump to:

Educational papers | Things we lead | Major contributors | Minor contributors

Educational papers we wrote {#ed-papers}

The Twenty Questions of Bioimage Object Analysis[2023] / Tool implementation

A biologist’s guide to planning and performing quantitative bioimaging experiments[2023]

Creating and troubleshooting microscopy analysis workflows: Common challenges and common solutions[2024]

A postdoctoral training program in bioimage analysis[2024]

Things we lead {#ed-lead}

The Scientific Community Image Forum - Forum.image.sc / Paper - Scientific Community Image Forum: A discussion forum for scientific image software[2019]

Imaging Platform Blog

YouTube tutorials

Office hours link

Ask Erin/Dear Beth image analysis tips video podcast / Resources from podcast episodes

COBA website

Bioimaging Guide - English / Spanish / Portuguese / Czech

The Image Based Profiling Handbook

The Cell Painting Gallery (image repository) - AWS RODA Website / Explore on Quilt / See featured on the National AI Ready Resource (NAIRR) Pilot website / Paper - Cell Painting Gallery: an open resource for image-based profiling[2024]

Bioimage Analysis Beginnings Broad Bootcamp (week-long paid course)

Things we are major (but far from sole) contributors to {#ed-major}

Global Bioimage Analysts' Society (GloBIAS) Global Registries - For bioimage analysts / For bioimage analysis trainers

Virtual I2K Meeting - 2023 edition / 2024 edition / 2025 edition

Things we contributed to (but others led) {#ed-minor}

Online guide for checklists for imaging and image analysis / Paper - Community-developed checklists for publishing images and image analysis [2023]

Metrics Reloaded online metric chooser / Paper 1 - Metrics reloaded: recommendations for image analysis validation[2024] / Paper 2 - Understanding metric-related pitfalls in image analysis validation[2024]

Microtutor imaging education platform

Boston BioImage Analysis Society (BostonBIAS) - sign up form

Cold Spring Harbor Quantitative Imaging Course (two-week paid course)

Papers you might find useful {#papers}

Full list of lab papers and preprints

Jump to:

Profiling | Tools and methods | Reviews | Community Surveys | Opinions/Perspectives

Profiling protocols and methods {#profiling}

Interpreting image-based profiles using Morpheus and single-cell visualization[2023]

Optimizing the Cell Painting assay for image-based profiling[2023]

A genome-wide atlas of human cell morphology[2024]

Live-cell painting: Image-based profiling in live cells using acridine orange[2025]

Alternate dyes for image-based profiling assays[2025]

Computational tools and methods {#tools}

ImageJ and CellProfiler: Complements in Open-Source Bioimage Analysis[2020]

Reproducible image-based profiling with Pycytominer[2025]

CellPhePy: A python implementation of the CellPhe toolkit for automated cell phenotyping from microscopy time-lapse videos[2025]

Reviews {#reviews}

Open-source deep-learning software for bioimage segmentation[2021]

Defining the boundaries: challenges and advances in identifying cells in microscopy images[2024]

Community Surveys {#surveys}

2020 BioImage Analysis Survey: Community experiences and needs for the future[2021]

Bridging Imaging Users to Imaging Analysis - A community survey[2023]

2024 survey results - forum post / repository

Opinions/Perspectives {#opinions}

Moving beyond the desktop: Prospects for Practical Bioimage Analysis via the Web[2023]

Building momentum through networks: Bioimaging across the Americas[2024]

The Crucial Role of BioImage Analysts in Scientific Research and Publication[2024]

A Call for FAIR and Open-Access Training Materials to advance Bioimage Analysis (preprint)[2024]

Harmonizing the Generation and Pre-publication Stewardship of FAIR Image Data (preprint)[2024]

Enabling Global Image Data Sharing in the Life Sciences [2025]

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