STLI is a Python command line app which was written to automate long term Time Lapse Digital Microscopy and Real-Time Thermosensing. This app was written first in June 2015 as a novice research project by fpcyan for use observing DNA Crystallization in Ned Seeman's DNA Nanotechnology Lab at New York University. The original Arduino code was written by Adafruit, and modified for parallel temperature sensing.
Apologies for the ugly code! Because I don't have access to a equipment for testing, I am cleaning it up slowly because stability > a well-needed rewrite.
The current projects are build using:
To use this repo, you need an Arduino Uno and MCP9808 12C temperature sensor (or two).
Click here for extensive instructions intended for a general audience. Click here for a brief explanation of the limitations of this analytical tool.
In brief:
- Set up your experiment under your microscope.
- upload the instructions in the provided Adafruit_MCP9808 library to the your Arduino.
- Verify that the active USB port is the same as in sensor_time_lapse_integration.py.
- Run sensor_time_lapse_integration.py, and make sure that it is watching whatever directory your microscope saves photos to.
- Run automate_time_lapse.ahk (default time lapse interval of 30min).
- Enjoy!