ggrant@pcbi.upenn.edu

Hello,

I am working on zebra fish RNA data and am using danRer10 reference genome for which the rum indexes are not present. I tried creating indexes using the create_indexes_from_ucsc.pl script but the throws errors like,

ERROR: exon for chr19:24577623-24578829 not found. chr19 - 24577622 24579426 2 24577622,24578908, 24578829,24579426, NM_001317760(danRer10_RefSeqgene) i=0 at rum-master/bin/create_indexes_from_ucsc.pl line 97.

I would be grateful if you help me in solving this issue or guide me for any other alternative which I can use.

Thanks!!