This repository contains the scripts and datasets used in the study:
"Comparative and systems analyses of Leishmania spp. non-coding RNAs through developmental stages"
Published in PLOS Neglected Tropical Diseases
https://doi.org/10.1371/journal.pntd.0013108
This project focuses on the identification, expression analysis, and functional inference of non-coding RNAs (ncRNAs) in multiple species of the Leishmania genus, using a combination of comparative genomics and transcriptomic profiling across key developmental stages.
The repository includes:
- Scripts for ncRNA prediction, filtering, and classification
- Pipelines for RNA-seq processing and expression quantification
- Co-expression for functional inference
- BED files with predicted ncRNA coordinates per species
- Supplementary materials supporting the manuscript
If you use this repository, please cite the following publication:
Martínez-Hernandez JE, Aliaga-Tobar V, González-Rosales C, Monte-Neto R, Martin AJM, Maracaja-Coutinho V. (2025).
Comparative and systems analyses of Leishmania spp. non-coding RNAs through developmental stages.
PLOS Neglected Tropical Diseases. https://doi.org/10.1371/journal.pntd.0013108
For questions or contributions, feel free to open an issue or contact the corresponding authors.