Questions regarding the thread settings of bcftools mpileup

[qxy647]

Hello Professor, I would like to ask you a question. During the process of generating gene locus information using bcftools mpileup, I gave the --threads 10 command. However, the number of background threads was always only 1-2 running. When I changed the parameter of this command, the number of threads did not change. I would like to ask what is the reason for this? The command is as follows.：bcftools mpileup --fasta-ref GCA_046118805.1_ASM4611880v1_genomic.fna n-b list.txt -C 50 -q 20 -Q 30 -m 2 -I --threads 10 | bcftools call -mv --threads 10 | bcftools filter --threads 10 -g 5 -G 3 -Oz -o ah.vcf.gz